Synchronization of cell division in microorganisms by percoll gradients.
نویسندگان
چکیده
We describe a method for obtaining synchronously dividing cells of bacteria (Escherichia coli B and K-12 and Bacillus subtilis 168) and fission yeasts (Schizosaccharomyces pombe) by the use of Percoll density gradients.
منابع مشابه
Buoyant density constancy of Schizosaccharomyces pombe cells.
Buoyant densities of cells from exponentially growing cultures of the fission yeast Schizosaccharomyces pombe 972h- with division rates from 0.14 to 0.5 per h were determined by equilibrium centrifugation in Percoll gradients. Buoyant densities were independent of growth rate, with an average value (+/- standard error) of 1.0945 (+/- 0.00037) g/ml. When cells from these cultures were separated ...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 144 1 شماره
صفحات -
تاریخ انتشار 1980